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The kit is based on multiplex amplification of 28 STR loci followed by separation of PCR products by capillary electrophoresis. Of these, 25 autosomal loci: D3S1358, D2S441, D1S1656, D19S433, TPOX, D2S1338, TH01, D16S539, CSF1PO, D18S51, D21S11, D22S1045, D8S1179, VWA, D5S818, FGA, D13S31 7, D7S820, D12S391, D10S1248, SE33, DYS391, D8S1132, D7S1517, Penta D, Penta E and 3 sex loci: Amelogenin, Yindel, DYS391. The STR loci presented in the set are highly polymorphic, which allows for reliable identification of a person. The set includes all loci from the CODIS (Combined DNA Index System) and ESS (European Standard Set) databases, as well as loci SE33, Penta D, Penta E. To obtain the complete (for all 28 STR loci) genotype of the test sample, 0.125 nanograms (ng) of non-degraded DNA is sufficient. The optimal amount of DNA introduced into the reaction is 1 ng. The range of DNA concentrations for amplification is from 0.1 to 4 ng per reaction.

Genta-ALP alkaline phosphatase thermolabile catalyzes the cleavage of 5’- and 3’-phosphate groups from DNA, RNA, oligonucleotides, nucleoside triphosphates, as well as dephosphorylation of proteins. Active in the PCR buffer. Isolated from a strain of Escherichia coli expressing the alkaline phosphatase gene from Gadus morua.

The specially formulated binding solution (GE) ensures effective cleaning with high yield. The kit is intended for scientific research only.
Benefits of the set:

• Yield 60-90%
• Indicators A260/280 = 1.80±0.05
• Purified DNA is suitable for ligation, restriction, transformation, sequencing, and PCR reactions.

Medicines may contain pyrogens, the sources of which can be bacteria, viruses, fungi, and pyrogens can also be by-products of the production process. Contamination of drugs with pyrogenic substances is a serious threat to the health of patients and can lead to the development of fever and, ultimately, shock and death. Currently, to determine the content of pyrogenic substances (bacterial endotoxins and substances of non-endotoxin nature), the “Pyrogenicity” test is used, carried out on rabbits, which is inaccurate and harmful to animals. The monocyte activation test (MAT) has higher sensitivity and does not require the use of animals.

The magnetic sorbent ExtraGen® is an aqueous suspension of solid-phase magnetic particles based on iron oxide and silicon oxide. It is designed to isolate nucleic acids from clinical and biological samples. The suspension of the magnetic sorbent has an "intermediate" sedimentation stability, which is especially important when using the material in automatic DNA isolation systems. ExtraGen® particles are resistant to heat, strong oxidants and intense mechanical influences (centrifugation, dispersion, etc.). The use of proprietary buffer solution allows the particles to be stored at room temperature for a year without deterioration of their sorption properties. As tests have shown, the ExtraGen® sorbent shows high efficiency in the isolation of nucleic acids both in manual mode and with the use of automatic stations.

Fusion DNA polymerase is a recombinant polypeptide consisting of a fusion of the thermostable DNA polymerase of Pyrococcus furiosus (Pfu) and the DNA binding protein of the thermophilic archaea species Sulfolobus solfataricus (Sso7d). The Sso7d protein binds to the minor groove of double-stranded DNA and further stabilizes the polymerase-template complex. Due to this, Fusion DNA polymerase has increased processivity, synthesis accuracy, fragment amplification rate, and increased resistance to PCR inhibitors compared to native Pfu DNA polymerase [1]. Fusion DNA polymerase has 5'→3' polymerase activity, 3'→5' exonuclease activity and synthesizes blunt-ended products.
Fusion DNA polymerase is a good choice for routine cloning and can be used to generate long or complex amplicons by PCR.
Examples of using Fusion DNA polymerase are presented at the end of the description.
Fusion DNA polymerase was isolated from an E. coli strain containing a plasmid with a cloned DNA fragment consisting of the fusion genes of the thermostable DNA polymerase of Pyrococcus furiosus (Pfu) and the DNA-binding protein of Sulfolobus solfataricus (Sso7d).
One unit of activity corresponds to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble DNA fraction in 30 min at 74°C.

A 5-fold reaction buffer that does not contain magnesium sulfate.

The kit “System for quantitative assessment of E. coli host DNA impurities by RT-PCR” is convenient and easy to use; it allows you to isolate residual DNA from a sample and amplify it using real-time PCR in the presence of the fluorescent dye SYBR Green I. The kit includes reagents for isolating DNA from nucleoprotein complexes and removing PCR inhibitors, a 2× reaction mixture BioMaster HS-qPCR SYBR Blue (2×), a standard DNA solution and a proven set of specific primers for the conserved region of E. coli 16S rRNA.

5x reaction buffer suitable for use in most PCR applications, including real-time PCR. If it is necessary to select the concentration of Mg2+ ions in the reaction mixture, it is recommended to use PCR buffers that do not contain magnesium salts. The standard 5x PCR buffer contains 10 mm MgSO4, which corresponds to 2 mM MgSO4 in the reaction mixture.

The kit uses the technology of DNA sorption on magnetic particles. The entire DNA extraction process combines the steps of sample lysis, subsequent selective binding of the released DNA to magnetic beads, and then, after a series of washes, elution with the option of concentrating the DNA in a low-salt buffer.
Benefits of the set:

• High yield of genomic DNA from E.coli and eukaryotic cells: 4-5 µg DNA from 0.5-1*109 bacterial cells and 0.25-1*106 eukaryotic cells.
• High yield of genomic DNA when isolated from various mouse tissues and organs.
• Indicators A260/280 = 1.80±0.05, A260/230 ≥ 2.1
• Purified DNA is verified to be free of proteins, nucleases or other contaminants and can be used in a wide range of molecular biology techniques such as PCR, RT-PCR or other enzyme reactions.
• High throughput for DNA extraction from large numbers of samples.

Genta-T4 DNA ligase is a recombinant enzyme that catalyzes the formation of a phosphodiester bond between the 5`-phosphate and 3`-hydroxyl end groups of double-stranded DNA. Isolated from a strain of Escherichia coli expressing the bacteriophage T4 DNA ligase gene. DNA ligase stitches both sticky and blunt ends, can repair single-strand breaks in double strands of DNA, RNA or DNA/RNA hybrids.

The product is a sterile 100 mM solution of the cap analogue m7GmAmG as an ammonium salt in water. The product is tested for the presence of endo- and exonuclease activity and is free from DNase and RNase contaminants. The purity of the nucleotide according to HPLC data is not less than 96%. Functional activity was confirmed in vitro in a transcription reaction.