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The dNTP mixture is an aqueous solution of four highly purified 2’-deoxynucleoside-5’-triphosphates (dATP, dTTP, dHTF, dCTF) in equimolar concentration

The kit is designed for isolating plasmid DNA from bacteria.
The operating principle of the kit is based on alkaline lysis of RNase-treated biomaterial, followed by selective binding of DNA from the clarified lysate on a siliconized column membrane. The colored solutions in the kit allow you to control the correct order of adding components, the completeness of lysis and neutralization.
Benefits of the set:

• Yield up to 25 µg from 2 ml of bacterial culture and up to 60* µg from 6-10 ml of bacterial culture
• Indicators A260/280 = 1.80±0.05, A260/230 ≥ 2.1
• Plasmid DNA has been verified to be suitable for restriction reactions, transformation, sequencing, PCR, transfection.

*product yield depends on the copy number of the plasmid, culture volume and cultivation conditions

Genta Bst DNA polymerase is a large fragment of Bacillus stearothermophilus DNA polymerase (a 67 kDa polypeptide). The enzyme has 5’-3’ polymerase activity, but does not have 5’-3’ and 3’-5’ exonuclease activity. Genta Bst DNA polymerase has a displacing activity and can be used for isothermal amplification of DNA in the LAMP/RT-LAMP format. The optimal temperature of enzyme activity is 60-65 °C.

The kit is based on multiplex amplification of 28 STR loci followed by separation of PCR products by capillary electrophoresis. Of these, 25 autosomal loci: D3S1358, D2S441, D1S1656, D19S433, TPOX, D2S1338, TH01, D16S539, CSF1PO, D18S51, D21S11, D22S1045, D8S1179, VWA, D5S818, FGA, D13S31 7, D7S820, D12S391, D10S1248, SE33, DYS391, D8S1132, D7S1517, Penta D, Penta E and 3 sex loci: Amelogenin, Yindel, DYS391. The STR loci presented in the set are highly polymorphic, which allows for reliable identification of a person. The set includes all loci from the CODIS (Combined DNA Index System) and ESS (European Standard Set) databases, as well as loci SE33, Penta D, Penta E. To obtain the complete (for all 28 STR loci) genotype of the test sample, 0.125 nanograms (ng) of non-degraded DNA is sufficient. The optimal amount of DNA introduced into the reaction is 1 ng. The range of DNA concentrations for amplification is from 0.1 to 4 ng per reaction.

A 5-fold reaction buffer that does not contain magnesium sulfate.

Fluorescent probes are an oligonucleotide with a fluorescent label and a suppressor that can bind to the DNA chain during amplification. When the probe is in a free state, both dyes are located close to each other, therefore, fluorescence quenching occurs. During PCR, at the stage of chain elongation, the hybridized probe is cleaved by a polymerase that has 5’-exonuclease activity, the dyes are separated, and a fluorescent signal proportional to the amount of the amplified product is observed. The fluorescence intensity increases in each cycle in proportion to the probe splitting rate. The use of probes in PCR makes it possible to increase the accuracy and selectivity of the reaction. A wide range of fluorescent dyes: FAM, R6G, VIC, HEX, JOE, TAMRA, ROX, Cy 5 and Cy5.5 The possibility of multiplex analysis of multiple DNA targets in one test tube. High efficiency of PCR-RV. Probe quality control: HPLC purification and mass spectrometry verification

Genta benzonuclease is a nuclease that cleaves all types of both DNA and RNA (single-stranded, double-stranded, linear and annular) to form oligodeoxynucleotides with terminal 5’-monophosphates 3-5 bases long. It is effective in a wide range of working conditions. It has no proteolytic activity. Isolated from a strain of Escherichia coli expressing the nuclease gene from the microorganism Serratia marcescens. Reaction conditions: 20 - 37 °C, optimal buffer: 50 mM Tris-HCl (pH 8.0 at 25 °C); 100 -150 mm NaCl; 1 mM MgCl2.

5X Genta Taq-AB qPCR master mix is a ready-to–use mixture for qualitative or quantitative PCR with the ability to detect results in real time. 5X Genta Taq-AB qPCR Master Mix contains all the components necessary for PCR, including Genta Taq-AB polymerase, dNTP, Mg2+, SYBR Green I dye and reaction buffer. To set up the PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. Inactivation of Genta Taq-AB polymerase with antibodies allows preparation of mixtures at room temperature. The "hot" start provides an increase in the specificity of amplification. The dissociation of the complex and the release of Genta Taq-AB DNA polymerase is provided by heating above 70 °C.

The kit “System for quantitative assessment of E. coli host DNA impurities by RT-PCR” is convenient and easy to use; it allows you to isolate residual DNA from a sample and amplify it using real-time PCR in the presence of the fluorescent dye SYBR Green I. The kit includes reagents for isolating DNA from nucleoprotein complexes and removing PCR inhibitors, a 2× reaction mixture BioMaster HS-qPCR SYBR Blue (2×), a standard DNA solution and a proven set of specific primers for the conserved region of E. coli 16S rRNA.

The product is a sterile 100 mM solution of the cap analogue m7GmAmG as an ammonium salt in water. The product is tested for the presence of endo- and exonuclease activity and is free from DNase and RNase contaminants. The purity of the nucleotide according to HPLC data is not less than 96%. Functional activity was confirmed in vitro in a transcription reaction.

5X Genta Taq-AB PCR Master mix is a ready-to–use mixture for qualitative or quantitative PCR with the ability to detect results in real time. 5X Genta Taq-AB PCR Master Mix contains all the components necessary for PCR, including Genta Taq-AB polymerase, dNTP, Mg2+ and reaction buffer. To set up the PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. Inactivation of Genta Taq-AB polymerase with antibodies allows preparation of mixtures at room temperature. The "hot" start provides an increase in the specificity of amplification. The dissociation of the complex and the release of Genta Taq-AB DNA polymerase is provided by heating above 70 °C.

Genta UDG thermolabile glycosylase is a thermosensitive enzyme that catalyzes the release of uracil from uracil-containing single- or double-stranded DNA. It is used to prevent the appearance of false positive results caused by contamination with amplicons. The enzyme does not show activity against RNA and oligomers and is fully incubated during the first PCR cycle when heated above 70 ° C, without interfering with the amplification of PCR products.