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Genta benzonuclease is a nuclease that cleaves all types of both DNA and RNA (single-stranded, double-stranded, linear and annular) to form oligodeoxynucleotides with terminal 5’-monophosphates 3-5 bases long. It is effective in a wide range of working conditions. It has no proteolytic activity. Isolated from a strain of Escherichia coli expressing the nuclease gene from the microorganism Serratia marcescens. Reaction conditions: 20 - 37 °C, optimal buffer: 50 mM Tris-HCl (pH 8.0 at 25 °C); 100 -150 mm NaCl; 1 mM MgCl2.

Genta RevM revertase (reverse transcriptase) is a genetically modified reverse transcriptase of the Moloney mouse leukemia virus (MMLV), designed for the synthesis of a complementary DNA chain (cDNA) on a single-stranded RNA matrix. Genta RevM was isolated from a strain of Escherichia coli expressing a modified MMLV reverse transcriptase gene. In comparison with the reverse transcriptase of the "wild type" Genta REV M, the revertase has increased thermal stability: the optimal temperature for the enzyme is 50 ° C, the enzyme remains active at temperatures up to 65 ° C. Reverse transcriptase Genta RevM revertase provides higher cDNA yield, allows longer fragments to be synthesized, has improved efficiency when using GC-rich RNA matrices, and also has higher productivity. The Genta RevM reverse transcriptase is suitable for RT-PCR in the "one-step" format.

Genta Bst DNA polymerase is a large fragment of Bacillus stearothermophilus DNA polymerase (a 67 kDa polypeptide). The enzyme has 5’-3’ polymerase activity, but does not have 5’-3’ and 3’-5’ exonuclease activity. Genta Bst DNA polymerase has a displacing activity and can be used for isothermal amplification of DNA in the LAMP/RT-LAMP format. The optimal temperature of enzyme activity is 60-65 °C.

The product is a sterile 10 mM solution of the ARCA cap structure analogue as an ammonium salt in water. The product is tested for the presence of endo- and exonuclease activity and is free from DNase and RNase contaminants. The purity of the nucleotide according to HPLC data is not less than 96%. Functional activity was confirmed in vitro in a transcription reaction.

5X Genta Taq-AB qPCR master mix is a ready-to–use mixture for qualitative or quantitative PCR with the ability to detect results in real time. 5X Genta Taq-AB qPCR Master Mix contains all the components necessary for PCR, including Genta Taq-AB polymerase, dNTP, Mg2+, SYBR Green I dye and reaction buffer. To set up the PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. Inactivation of Genta Taq-AB polymerase with antibodies allows preparation of mixtures at room temperature. The "hot" start provides an increase in the specificity of amplification. The dissociation of the complex and the release of Genta Taq-AB DNA polymerase is provided by heating above 70 °C.

Buffer for storage and dilution of reverse transcriptase.

The protocol is based on a modified method of alkaline lysis of a bacterial culture without preliminary sedimentation of bacteria, followed by selective binding of DNA from the clarified lysate on a siliconized column membrane. The technology makes it possible to speed up the isolation process due to rapid lysis directly from the biomass with treatment of the lysate with RNase A and further centrifugation.
Benefits of the set:
Yield up to 10 µg from 0.6 ml bacterial culture Indicators A260/280 = 1.80±0.05, A260/230 ≥ 2.1 It has been verified that plasmid DNA is suitable for restriction reactions, transformation, sequencing, PCR

A 5-fold reaction buffer suitable for use in the reaction of isothermal amplification of nucleic acids in LAMP and OT-LAMP formats. The standard 5x Genta LAMP buffer contains 25 mm MgSO4, which corresponds to 5 mM MgSO4 in the reaction mixture.

10-fold buffer for the ligation reaction, compatible with T4 DNA ligase.

Genta UDG thermolabile glycosylase is a thermosensitive enzyme that catalyzes the release of uracil from uracil-containing single- or double-stranded DNA. It is used to prevent the appearance of false positive results caused by contamination with amplicons. The enzyme does not show activity against RNA and oligomers and is fully incubated during the first PCR cycle when heated above 70 ° C, without interfering with the amplification of PCR products.

Oligonucleotides are short fragments of nucleic acids that are widely used in various fields of DNA diagnostics (medical diagnostics, forensic analysis, veterinary medicine, food quality control, etc.). Recently, oligonucleotides have been widely used as medicines against various genetic and other diseases. GenTerra produces DNA and RNA oligonucleotides on a scale of several milligrams to gram quantities, including modified oligonuleotides, fluorescently labeled oligonucleotides and real-time PCR probes.

The set of reagents can be used in research, clinical diagnostic and testing laboratories to decipher the sequences of fragments of single- or double-stranded plasmids, PCR products from 50 to 1000 in length nucleotides.