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10-fold buffer for the ligation reaction, compatible with T4 DNA ligase.

The protocol is based on a modified method of alkaline lysis of a bacterial culture without preliminary sedimentation of bacteria, followed by selective binding of DNA from the clarified lysate on a siliconized column membrane. The technology makes it possible to speed up the isolation process due to rapid lysis directly from the biomass with treatment of the lysate with RNase A and further centrifugation.
Benefits of the set:
Yield up to 10 µg from 0.6 ml bacterial culture Indicators A260/280 = 1.80±0.05, A260/230 ≥ 2.1 It has been verified that plasmid DNA is suitable for restriction reactions, transformation, sequencing, PCR

The polymer provides single-nucleotide separation of fragments of the Sanger DNA sequencing reaction in the range from 20 to 1000 nucleotides as part of genetic analyzers operating on the principle of capillary gel electrophoresis, for example, Nanofor 05

The specially formulated binding solution (GE) ensures effective cleaning with high yield. The kit is intended for scientific research only.
Benefits of the set:

• Yield 60-90%
• Indicators A260/280 = 1.80±0.05
• Purified DNA is suitable for ligation, restriction, transformation, sequencing, and PCR reactions.

Oligonucleotides are short fragments of nucleic acids that are widely used in various fields of DNA diagnostics (medical diagnostics, forensic analysis, veterinary medicine, food quality control, etc.). Recently, oligonucleotides have been widely used as medicines against various genetic and other diseases. GenTerra produces DNA and RNA oligonucleotides on a scale of several milligrams to gram quantities, including modified oligonuleotides, fluorescently labeled oligonucleotides and real-time PCR probes.

Genta TaqF DNA polymerase is a chemically modified recombinant analog of DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme has 5’-3’ polymerase activity and 5’-3’ exonuclease activity, but does not have 3’-5’ corrective exonuclease activity. The recombinant enzyme Genta TaqF DNA polymerase is suitable for use in routine PCR, including real-time PCR. Genta TaqF DNA polymerase is inactivated by chemical modification, which allows to prepare the reaction mixture at room temperature. The presence of a "hot start" increases the specificity and sensitivity of amplification. Polymerase activation occurs when it is heated to 95 ° C for 15 minutes.

ExoGen is a ready-to-use mixture of Genta-ExoI exonuclease I enzymes and the thermolabile alkaline phosphatase Genta-ALP in a reaction buffer. ExoGen mixture is used for enzymatic hydrolysis of an excessive amount of primers and nucleotides in PCR products (amplicons). The PCR products purified in this way are suitable for further use in various biological applications, such as cloning and DNA sequencing.

5x Genta TE is a buffer for the dilution of nucleic acids. Composition: 10 mM Tris*HCl, 1 mM EDTA, pH 8,0. Prepared with ultrapure water.

Genta-T4 DNA ligase is a recombinant enzyme that catalyzes the formation of a phosphodiester bond between the 5`-phosphate and 3`-hydroxyl end groups of double-stranded DNA. Isolated from a strain of Escherichia coli expressing the bacteriophage T4 DNA ligase gene. DNA ligase stitches both sticky and blunt ends, can repair single-strand breaks in double strands of DNA, RNA or DNA/RNA hybrids.

Genta-ALP alkaline phosphatase thermolabile catalyzes the cleavage of 5’- and 3’-phosphate groups from DNA, RNA, oligonucleotides, nucleoside triphosphates, as well as dephosphorylation of proteins. Active in the PCR buffer. Isolated from a strain of Escherichia coli expressing the alkaline phosphatase gene from Gadus morua.

5X Genta Single-tube RT-qPCR Master Mix is a ready-to–use mixture for conducting a cDNA synthesis reaction followed by quantitative and/or qualitative PCR with the possibility of detecting results in real time (RT-PCR-RV). 5X Genta Single-tube RT-qPCR Master Mix contains all the components necessary for RT-PCR, including Genta REV M reverse transcriptase, Genta TaqF DNA polymerase, dNTP, Mg2+, SYBR Green I dye and reaction buffer. To set up the RT-PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. The chemically inactivated DNA polymerase Genta TaqF allows to perform preparatory work at room temperature and provides highly specific amplification due to the possibility of a "hot" start. Inhibition of enzyme activity is removed when it is heated at 95 ° C for 15 minutes.

5X Genta Single-tube RT-PCR Master Mix is a ready-to–use mixture for conducting a cDNA synthesis reaction followed by quantitative and/or qualitative PCR with the possibility of detecting results in real time (RT-PCR-RV). 5X Genta Single-tube RT-PCR Master Mix contains all the components necessary for RT-PCR, including Genta REV M reverse transcriptase, Genta TaqF DNA polymerase, dNTP, Mg2+ and reaction buffer. To set up the RT-PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. The chemically inactivated DNA polymerase Genta TaqF allows for preparatory work at room temperature and provides highly specific amplification due to the possibility of a "hot" start. Inhibition of enzyme activity is removed when it is heated at 95 ° C for 15 minutes.