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44 products
D-Plants kit for DNA extraction from plants D-Plants-10
D-Plants kit for DNA extraction from plants D-Plants-10
from 3 150 ₽
1 supp.
The kit is intended for the extraction and purification of DNA from the following samples: 1. Leaves, needles, stamens, green parts of plants 2. Roots, stems, bark 3. Fruits, berries, seeds 4. Mosses, lichens 5. Unicellular algae
BIOLABMIKS
Novosibirsk
Produced in: Novosibirsk
5x Genta PCR Buffer
5x Genta PCR Buffer
from 78 ₽
5x reaction buffer suitable for use in most PCR applications, including real-time PCR. If it is necessary to select the concentration of Mg2+ ions in the reaction mixture, it is recommended to use PCR buffers that do not contain magnesium salts. The standard 5x PCR buffer contains 10 mm MgSO4, which corresponds to 2 mM MgSO4 in the reaction mixture.
Produced in: Moscow
Genta-B buffer 1
Genta-B buffer 1
from 78 ₽
Buffer for storage and dilution of DNA polymerase.
Produced in: Moscow
Similar to the ARCA cap structure
Similar to the ARCA cap structure
from 24 000 ₽
1 supp.
The product is a sterile 10 mM solution of the ARCA cap structure analogue as an ammonium salt in water. The product is tested for the presence of endo- and exonuclease activity and is free from DNase and RNase contaminants. The purity of the nucleotide according to HPLC data is not less than 96%. Functional activity was confirmed in vitro in a transcription reaction.
BIOLABMIKS
Novosibirsk
Produced in: Novosibirsk
Genta gDNA Isolation kit, KI-GgDNA
Genta gDNA Isolation kit, KI-GgDNA
from 10 000 ₽
The kit uses the technology of DNA sorption on magnetic particles. The entire DNA extraction process combines the steps of sample lysis, subsequent selective binding of the released DNA to magnetic beads, and then, after a series of washes, elution with the option of concentrating the DNA in a low-salt buffer.
Benefits of the set:
  • High yield of genomic DNA from E.coli and eukaryotic cells: 4-5 µg DNA from 0.5-1*109 bacterial cells and 0.25-1*106 eukaryotic cells.
  • High yield of genomic DNA when isolated from various mouse tissues and organs.
  • Indicators A260/280 = 1.80±0.05, A260/230 ≥ 2.1
  • Purified DNA is verified to be free of proteins, nucleases or other contaminants and can be used in a wide range of molecular biology techniques such as PCR, RT-PCR or other enzyme reactions.
  • High throughput for DNA extraction from large numbers of samples.
Produced in: Moscow
5X Genta qPCR Master mix, 250 reactions
5X Genta qPCR Master mix, 250 reactions
from 1 600 ₽
5X Genta qPCR Master Mix is a ready-to–use mixture for qualitative or quantitative PCR with real-time detection of results. The master mix ensures reliable PCR operation in a wide range of applications. The master mix allows PCR-RV to be carried out in a multiplex format. 5X Genta qPCR Master mix contains all the components necessary for PCR, including Genta TaqF polymerase, dNTP, Mg2+, SYBR Green I dye and reaction buffer. To set up the PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. The chemically inactivated DNA polymerase Genta TaqF allows to perform preparatory work at room temperature and provides highly specific amplification due to the possibility of a "hot" start. Inhibition of enzyme activity is removed when it is heated at 95 ° C for 15 minutes.
Produced in: Moscow
Genta Taq-AB DNA Polymerase, 5,000 units/ml
Genta Taq-AB DNA Polymerase, 5,000 units/ml
from 2 300 ₽
Genta Taq-AB DNA polymerase is a recombinant analog of DNA polymerase from thermophilic bacterium Thermus aquaticus inactivated by specific monoclonal antibodies. The enzyme has 5’-3’ polymerase activity and 5’-3’ exonuclease activity, but does not have 3’-5’ corrective exonuclease activity. The recombinant enzyme Genta Taq-AB DNA polymerase is suitable for use in routine PCR, including real-time PCR. Inactivation with monoclonal antibodies allows to prepare the reaction mixture at room temperature. The presence of a "hot start" increases the specificity and sensitivity of amplification. Dissociation of the complex with antibodies and activation of Genta Taq-AB DNA polymerase occurs when it is heated above 70 °C.
Produced in: Moscow
Genta REV M Revertase, 100,000 units/ml
Genta REV M Revertase, 100,000 units/ml
from 3 500 ₽
Genta RevM revertase (reverse transcriptase) is a genetically modified reverse transcriptase of the Moloney mouse leukemia virus (MMLV), designed for the synthesis of a complementary DNA chain (cDNA) on a single-stranded RNA matrix. Genta RevM was isolated from a strain of Escherichia coli expressing a modified MMLV reverse transcriptase gene. In comparison with the reverse transcriptase of the "wild type" Genta REV M, the revertase has increased thermal stability: the optimal temperature for the enzyme is 50 ° C, the enzyme remains active at temperatures up to 65 ° C. Reverse transcriptase Genta RevM revertase provides higher cDNA yield, allows longer fragments to be synthesized, has improved efficiency when using GC-rich RNA matrices, and also has higher productivity. The Genta RevM reverse transcriptase is suitable for RT-PCR in the "one-step" format.
Produced in: Moscow
GentaMAG-NK nucleic acid secretion kit, 100 secretions
GentaMAG-NK nucleic acid secretion kit, 100 secretions
from 2 500 ₽
a set of reagents for the secretion of nucleic acids (DNA/RNA) from biological material by sorption on magnetic particles. Compatible biomaterial: • Smears from the upper respiratory tract • Buccal epithelium • Saliva • Fecal extracts • Whole blood • Blood plasma
Produced in: Moscow
Fusion DNA polymerase (Pfu-Sso7d) 100 u.a.
Fusion DNA polymerase (Pfu-Sso7d) 100 u.a.
from 4 200 ₽
1 supp.
Fusion DNA polymerase is a recombinant polypeptide consisting of a fusion of the thermostable DNA polymerase of Pyrococcus furiosus (Pfu) and the DNA binding protein of the thermophilic archaea species Sulfolobus solfataricus (Sso7d). The Sso7d protein binds to the minor groove of double-stranded DNA and further stabilizes the polymerase-template complex. Due to this, Fusion DNA polymerase has increased processivity, synthesis accuracy, fragment amplification rate, and increased resistance to PCR inhibitors compared to native Pfu DNA polymerase [1]. Fusion DNA polymerase has 5'→3' polymerase activity, 3'→5' exonuclease activity and synthesizes blunt-ended products.
Fusion DNA polymerase is a good choice for routine cloning and can be used to generate long or complex amplicons by PCR.
Examples of using Fusion DNA polymerase are presented at the end of the description.
Fusion DNA polymerase was isolated from an E. coli strain containing a plasmid with a cloned DNA fragment consisting of the fusion genes of the thermostable DNA polymerase of Pyrococcus furiosus (Pfu) and the DNA-binding protein of Sulfolobus solfataricus (Sso7d).
One unit of activity corresponds to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble DNA fraction in 30 min at 74°C.
BIOLABMIKS
Novosibirsk
Reagent set «ALPYR MAT»
Reagent set «ALPYR MAT»
1 supp.
Medicines may contain pyrogens, the sources of which can be bacteria, viruses, fungi, and pyrogens can also be by-products of the production process. Contamination of drugs with pyrogenic substances is a serious threat to the health of patients and can lead to the development of fever and, ultimately, shock and death. Currently, to determine the content of pyrogenic substances (bacterial endotoxins and substances of non-endotoxin nature), the “Pyrogenicity” test is used, carried out on rabbits, which is inaccurate and harmful to animals. The monocyte activation test (MAT) has higher sensitivity and does not require the use of animals.
Produced in: Moscow
Reagent set «ALPYR Test»
Reagent set «ALPYR Test»
The “ALPYR Test” reagent set is intended for carrying out the LAL test using the gel-thromb test method and is designed for 300 determinations, which means 36–40 samples using the qualitative method (method A) or 15–18 samples using the quantitative method (method B). The kit includes reagents and basic auxiliary materials for analysis.
Each item in the kit comes with a quality passport or certificate of analysis.
Produced in: Moscow