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Medicines may contain pyrogens, the sources of which can be bacteria, viruses, fungi, and pyrogens can also be by-products of the production process. Contamination of drugs with pyrogenic substances is a serious threat to the health of patients and can lead to the development of fever and, ultimately, shock and death. Currently, to determine the content of pyrogenic substances (bacterial endotoxins and substances of non-endotoxin nature), the “Pyrogenicity” test is used, carried out on rabbits, which is inaccurate and harmful to animals. The monocyte activation test (MAT) has higher sensitivity and does not require the use of animals.

The protocol is based on a modified method of alkaline lysis of a bacterial culture without preliminary sedimentation of bacteria, followed by selective binding of DNA from the clarified lysate on a siliconized column membrane. The technology makes it possible to speed up the isolation process due to rapid lysis directly from the biomass with treatment of the lysate with RNase A and further centrifugation.
Benefits of the set:
Yield up to 10 µg from 0.6 ml bacterial culture Indicators A260/280 = 1.80±0.05, A260/230 ≥ 2.1 It has been verified that plasmid DNA is suitable for restriction reactions, transformation, sequencing, PCR