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A 5-fold reaction buffer that does not contain magnesium sulfate.

The dNTP mixture is an aqueous solution of four highly purified 2’-deoxynucleoside-5’-triphosphates (dATP, dTTP, dHTF, dCTF) in equimolar concentration

Genta Taq-AB DNA polymerase is a recombinant analog of DNA polymerase from thermophilic bacterium Thermus aquaticus inactivated by specific monoclonal antibodies. The enzyme has 5’-3’ polymerase activity and 5’-3’ exonuclease activity, but does not have 3’-5’ corrective exonuclease activity. The recombinant enzyme Genta Taq-AB DNA polymerase is suitable for use in routine PCR, including real-time PCR. Inactivation with monoclonal antibodies allows to prepare the reaction mixture at room temperature. The presence of a "hot start" increases the specificity and sensitivity of amplification. Dissociation of the complex with antibodies and activation of Genta Taq-AB DNA polymerase occurs when it is heated above 70 °C.

ExtraGen®Black is a series of stable paramagnetic particles, ideal for the isolation of nucleic acids. ExtraGen® Black has a greater binding capacity compared to ExtraGen®, has a high binding efficiency with nucleic acids, is light and easy to use. ExtraGen® Black particles are stable under various conditions, which is especially important at the stages of sample preparation, in particular at the lysis stage. Characteristics and advantages: There is no need for a centrifuge and plastic columns High purity and speed of extraction Compatible with lysis/sequencing reagents and automated equipment

Genta-T4 DNA ligase is a recombinant enzyme that catalyzes the formation of a phosphodiester bond between the 5`-phosphate and 3`-hydroxyl end groups of double-stranded DNA. Isolated from a strain of Escherichia coli expressing the bacteriophage T4 DNA ligase gene. DNA ligase stitches both sticky and blunt ends, can repair single-strand breaks in double strands of DNA, RNA or DNA/RNA hybrids.

Genta UDG thermolabile glycosylase is a thermosensitive enzyme that catalyzes the release of uracil from uracil-containing single- or double-stranded DNA. It is used to prevent the appearance of false positive results caused by contamination with amplicons. The enzyme does not show activity against RNA and oligomers and is fully incubated during the first PCR cycle when heated above 70 ° C, without interfering with the amplification of PCR products.

Genta benzonuclease is a nuclease that cleaves all types of both DNA and RNA (single-stranded, double-stranded, linear and annular) to form oligodeoxynucleotides with terminal 5’-monophosphates 3-5 bases long. It is effective in a wide range of working conditions. It has no proteolytic activity. Isolated from a strain of Escherichia coli expressing the nuclease gene from the microorganism Serratia marcescens. Reaction conditions: 20 - 37 °C, optimal buffer: 50 mM Tris-HCl (pH 8.0 at 25 °C); 100 -150 mm NaCl; 1 mM MgCl2.

Genta Bst DNA polymerase is a large fragment of Bacillus stearothermophilus DNA polymerase (a 67 kDa polypeptide). The enzyme has 5’-3’ polymerase activity, but does not have 5’-3’ and 3’-5’ exonuclease activity. Genta Bst DNA polymerase has a displacing activity and can be used for isothermal amplification of DNA in the LAMP/RT-LAMP format. The optimal temperature of enzyme activity is 60-65 °C.

5x reaction buffer suitable for use in most PCR applications, including real-time PCR. If it is necessary to select the concentration of Mg2+ ions in the reaction mixture, it is recommended to use PCR buffers that do not contain magnesium salts. The standard 5x PCR buffer contains 10 mm MgSO4, which corresponds to 2 mM MgSO4 in the reaction mixture.

Genta-ExoI exonuclease I hydrolyzes single-stranded DNA in the 3'→ 5' direction, releasing deoxyribonucleoside 5'-monophosphate. It does not cleave DNA chains whose 3’-end is blocked by a phosphoryl or acetyl group. Active in the PCR buffer. Isolated from a strain of Escherichia coli expressing the exonuclease I gene of E.coli.

A 5-fold reaction buffer suitable for use in the reaction of isothermal amplification of nucleic acids in LAMP and OT-LAMP formats. The standard 5x Genta LAMP buffer contains 25 mm MgSO4, which corresponds to 5 mM MgSO4 in the reaction mixture.

The protocol is based on a modified method of alkaline lysis of a bacterial culture without preliminary sedimentation of bacteria, followed by selective binding of DNA from the clarified lysate on a siliconized column membrane. The technology makes it possible to speed up the isolation process due to rapid lysis directly from the biomass with treatment of the lysate with RNase A and further centrifugation.
Benefits of the set:
Yield up to 10 µg from 0.6 ml bacterial culture Indicators A260/280 = 1.80±0.05, A260/230 ≥ 2.1 It has been verified that plasmid DNA is suitable for restriction reactions, transformation, sequencing, PCR