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Genta Bst DNA polymerase is a large fragment of Bacillus stearothermophilus DNA polymerase (a 67 kDa polypeptide). The enzyme has 5’-3’ polymerase activity, but does not have 5’-3’ and 3’-5’ exonuclease activity. Genta Bst DNA polymerase has a displacing activity and can be used for isothermal amplification of DNA in the LAMP/RT-LAMP format. The optimal temperature of enzyme activity is 60-65 °C.

Fluorescent probes are an oligonucleotide with a fluorescent label and a suppressor that can bind to the DNA chain during amplification. When the probe is in a free state, both dyes are located close to each other, therefore, fluorescence quenching occurs. During PCR, at the stage of chain elongation, the hybridized probe is cleaved by a polymerase that has 5’-exonuclease activity, the dyes are separated, and a fluorescent signal proportional to the amount of the amplified product is observed. The fluorescence intensity increases in each cycle in proportion to the probe splitting rate. The use of probes in PCR makes it possible to increase the accuracy and selectivity of the reaction. A wide range of fluorescent dyes: FAM, R6G, VIC, HEX, JOE, TAMRA, ROX, Cy 5 and Cy5.5 The possibility of multiplex analysis of multiple DNA targets in one test tube. High efficiency of PCR-RV. Probe quality control: HPLC purification and mass spectrometry verification

A 5-fold reaction buffer suitable for use in the reaction of isothermal amplification of nucleic acids in LAMP and OT-LAMP formats. The standard 5x Genta LAMP buffer contains 25 mm MgSO4, which corresponds to 5 mM MgSO4 in the reaction mixture.

5X Genta Taq-AB qPCR master mix is a ready-to–use mixture for qualitative or quantitative PCR with the ability to detect results in real time. 5X Genta Taq-AB qPCR Master Mix contains all the components necessary for PCR, including Genta Taq-AB polymerase, dNTP, Mg2+, SYBR Green I dye and reaction buffer. To set up the PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. Inactivation of Genta Taq-AB polymerase with antibodies allows preparation of mixtures at room temperature. The "hot" start provides an increase in the specificity of amplification. The dissociation of the complex and the release of Genta Taq-AB DNA polymerase is provided by heating above 70 °C.

Genta TaqF DNA polymerase is a chemically modified recombinant analog of DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme has 5’-3’ polymerase activity and 5’-3’ exonuclease activity, but does not have 3’-5’ corrective exonuclease activity. The recombinant enzyme Genta TaqF DNA polymerase is suitable for use in routine PCR, including real-time PCR. Genta TaqF DNA polymerase is inactivated by chemical modification, which allows to prepare the reaction mixture at room temperature. The presence of a "hot start" increases the specificity and sensitivity of amplification. Polymerase activation occurs when it is heated to 95 ° C for 15 minutes.

5X Genta qPCR Master Mix is a ready-to–use mixture for qualitative or quantitative PCR with real-time detection of results. The master mix ensures reliable PCR operation in a wide range of applications. The master mix allows PCR-RV to be carried out in a multiplex format. 5X Genta qPCR Master mix contains all the components necessary for PCR, including Genta TaqF polymerase, dNTP, Mg2+, SYBR Green I dye and reaction buffer. To set up the PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. The chemically inactivated DNA polymerase Genta TaqF allows to perform preparatory work at room temperature and provides highly specific amplification due to the possibility of a "hot" start. Inhibition of enzyme activity is removed when it is heated at 95 ° C for 15 minutes.