Search

It is intended for the preparation of samples in the form of compressed tablets consisting of the test substance in a mixture with microcrystalline potassium bromide (KBr). The mold is used in combination with the hydraulic press GP 200-13. - the mold is made of high-quality alloy hardened steel - to obtain better compressed tablets with KBr, the mold can be pumped out using a pre-vacuum pump. The customer can purchase the pump independently, or order it from the NPF “SIMEX”. Pumping out the mold is not necessary for most studies. - the kit includes a special dispenser for placing the optimal amount of the pressed mixture in the mold - to install the finished samples in the spectrometer, a holder of KBr tablets and D13mm substrate windows is used

Field of application: -Gene expression analysis -One-step standard RT-PCR -One-step standard RT-PCR and the use of fluorescent probes -One-step real-time RT-PCR with SYBR Green I fluorescent dye Advantages of use: -High specificity -High sensitivity -Easy and convenient to use -Low pipetting and cross-contamination errors -Standard conditions for the same type of reactions -The possibility of TA cloning of PCR products due to the amplified DNA fragments of deoxyadenosine residues protruding at the ends.

It allows recording transmission spectra of objects with sizes from 500 microns (precious stones, optical parts, semiconductor materials). It is also used in the study of samples compressed into small-diameter tablets with KBr (the method is convenient for spectral analysis of powdery substances - the prefix allows you to work with small amounts of the sample using a manual press for the manufacture of tablets). • The diameter of the focus spot is less than 3 mm • The design uses parabolic mirror optics • The set-top box is equipped with a set of replaceable diaphragm holders for samples of different sizes • Windows-substrates made of IR-transparent materials can be placed in the holder after pasty, liquid substances or extracts are applied to them, followed by drying of the layers • The slide table has the possibility of smooth movement in vertical and horizontal directions to achieve the maximum signal level

The sample heating system is designed to expand the capabilities of standard set-top boxes NPVO-ZDO and NPVO-A. It consists of a heated replaceable frame with a diamond prism and a temperature controller unit. For most solid samples in the heated state, it is possible to obtain a much more pronounced NPV spectrum than in the cold state. This makes it possible to increase the sensitivity of the method several times. The properties of some substances and compounds change dramatically with an increase in temperature, chemical interaction processes between components, their decomposition, oxidation in air, etc. may occur. If changes in the chemical composition of a substance occur during heating, a set of spectra corresponding to the kinetics of the process can be obtained with the help of an NPVO prefix with a diamond thermocell and a temperature controller, which is essential expands the possibilities of the method in research. Technical characteristics of the NPVO set-top box with a thermocell and a controller Transmission in the operating range of the spectrum, % of the input signal at least 10 The recommended number of scans when registering spectra is 25 Spectrum registration time at 25 scans (resolution 4 cm-1), sec 30 The depth of penetration of radiation into the sample, microns 5 – 15 Minimum dimensions of the solid sample, mm 0.5 × 0.5 Minimum volume of the liquid under study, ml 1 Minimum dimensions of the fiber sample: cross-section diameter/length, mm 0.3/1 The material of the crystal substrate is DIAMOND The diameter of the free zone of the crystal, mm not less than 2 x 3 The diameter of the focus spot, mm 1.5 Maximum temperature With 220o Adjustment accuracy, With 1o Time to reach the maximum temperature, min 10 Overall dimensions, mm 130×200×90 Weight, kg 1,2

The spectrometer was created according to the Cherni-Turner scheme based on a flat diffraction grating and an MAES analyzer with one line of photodetectors. It has increased photometric accuracy due to cooling and temperature stabilization of the photodetector range, as well as due to its shell-less design, in which there is no re-reflection of radiation on the cover glass and the background level in the recorded spectrum decreases. The optical scheme and design of the spectrometer are optimized to obtain a high-quality spectrum with a low level of background radiation in any of the regions lying in the spectral range of 190-1100 nm. The choice of the working area is carried out by changing and rotating the diffraction gratings. The sealed case of the spectrometer is filled with an inert gas. The radiation is injected into the spectrometer using a quartz capacitor or a fiber-optic cable with an SMA-905 connector. The optical scheme and design of the spectrometer are protected by patents.

The MAES analyzer is a modern means of measuring the intensities of spectral lines and then calculating the concentrations of the elements to be determined.

The flame spectrometer is designed for rapid determination of a wide range of concentrations (up to 8 orders of magnitude) of sodium, lithium, potassium, calcium, barium, caesium, rubidium in technological solutions. The excitation of atoms occurs in an air-acetylene flame. The device consists of a three-slit burner with flame control, a pneumatic sprayer, a spray chamber, an optical radiation input system into the "Hummingbird-2" spectrometer and an automatic air and acetylene supply system, with the possibility to control and adjust gas flow. The use of a three-slit burner provides increased flame temperature over the central slit of the burner due to the external flame layers. This makes it possible to determine low concentrations of calcium and barium. At the same time, it remains the possibility to determine impurities in highly concentrated solutions without clogging the burner slots. The lighting system of the spectrometer is mirror-lens, thanks to this, radiation collected from both sides of the burner is introduced into the polychromator.

The spectrometer is designed to perform quantitative and qualitative spectral analysis of various substances and materials (powders, metals, solutions) in factory and research laboratories. The spectrometer is compact due to the vertical arrangement of the Paschen-Runge optical system. It consists of two MAES analyzers with 10 lines of photodiodes installed on a circle with a radius of 520 mm, a non-classical concave diffraction grating, a special table with a built-in computer and a spectrum excitation source.

Designed for the preparation of samples in the form of compressed tablets with a diameter of 3.5 mm, consisting of the test substance in a mixture with microcrystalline potassium bromide (KBr). - the principle of operation of the device is based on creating the pressure necessary for pressing due to a mechanical lever system - the press is made of high-quality alloy hardened steel - for the manufacture of a tablet with a diameter of 3.5 mm, a minimum amount of the test substance and KBr is required - for the study of finished samples on a Fourier spectrometer, a vertical or horizontal microfocusing prefix is used

It is designed for measurement by the method of disturbed total internal reflection with simultaneous visualization of a micro-object on an integrated and external monitor, as well as by the method of mirror-diffuse reflection with an angle of incidence of 45o at the upper location of the sample. The prefix in the mode of a single NIP is used to register absorption spectra: liquids of any degree of viscosity (solutions, suspensions, oils, etc.), including those with high chemical activity; solid objects of arbitrary shape, including samples with very high hardness (any polymers, fragments of paint coatings, etc.); powdery substances, including powders with very high hardness (drugs, pharmaceuticals, explosives, inorganic compounds); samples in the form of thin tapes; samples in the form of fibers. The maximum hardness and chemical resistance of the diamond significantly expand the possibilities of the method; there is no need for periodic replacement of the crystal. The set-top box allows you to register spectra without time–consuming sample preparation, and the presence of a visual inspection system of the studied surface with a high-quality video camera and a built-in high-definition mini-monitor increases efficiency when working with small samples - fragments of thin fibers, microparticles, etc. The built-in monitor has the functions of digital 10X zoom, inverting, etc. The image can be simultaneously displayed on the computer screen (using a USB interface) and then saved as a file. The removable flange provides a quick and convenient sample change and cleaning of the crystal surface. The design with a diamond element protruding above the base plane of the NPVO allows you to study samples with sufficiently large overall dimensions. The high quality and repeatability of the results is achieved due to the absence of the influence of the thickness of the substance layer on the shape of the spectrum and the intensity of the absorption bands. The sample retains its original physicochemical properties and, if necessary, can be further investigated by other methods. The universal attachment clamp is equipped with a precision lever mechanism for quickly lowering the tip and a micrometer screw that allows you to pre–set the optimal degree of pressure - this ensures quick sample change and repeatability of the results during measurements. For convenience when working with liquid and paste-like samples, as well as in the ZDO mode, it is possible to rotate the clamping console by 180o. The console is equipped with two replaceable tips – with a spherical working part and with a flat hinged head. The high hardness of the diamond allows the use of large clamping forces, which is the determining factor for obtaining high-quality spectra. A replacement table is used to register the specular and diffuse reflection spectra. The sample is located on the subject plane of the investigated surface downwards. The method is used to determine the spectral characteristics of optical parts, thin tapes on the surface, crystals, and other large solid objects of arbitrary shape and size.

Field of application: - Real-time loop isothermal amplification with Using SYBR Green I intercalating dye - loop isothermal amplification with endpoint detection.