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Genta Taq-AB DNA polymerase is a recombinant analog of DNA polymerase from thermophilic bacterium Thermus aquaticus inactivated by specific monoclonal antibodies. The enzyme has 5’-3’ polymerase activity and 5’-3’ exonuclease activity, but does not have 3’-5’ corrective exonuclease activity. The recombinant enzyme Genta Taq-AB DNA polymerase is suitable for use in routine PCR, including real-time PCR. Inactivation with monoclonal antibodies allows to prepare the reaction mixture at room temperature. The presence of a "hot start" increases the specificity and sensitivity of amplification. Dissociation of the complex with antibodies and activation of Genta Taq-AB DNA polymerase occurs when it is heated above 70 °C.

The magnetic sorbent ExtraGen® is an aqueous suspension of solid-phase magnetic particles based on iron oxide and silicon oxide. It is designed to isolate nucleic acids from clinical and biological samples. The suspension of the magnetic sorbent has an "intermediate" sedimentation stability, which is especially important when using the material in automatic DNA isolation systems. ExtraGen® particles are resistant to heat, strong oxidants and intense mechanical influences (centrifugation, dispersion, etc.). The use of proprietary buffer solution allows the particles to be stored at room temperature for a year without deterioration of their sorption properties. As tests have shown, the ExtraGen® sorbent shows high efficiency in the isolation of nucleic acids both in manual mode and with the use of automatic stations.

5x Genta TE is a buffer for the dilution of nucleic acids. Composition: 10 mM Tris*HCl, 1 mM EDTA, pH 8,0. Prepared with ultrapure water.

Release form 1:

• – a set of reagents for manual RNA isolation and at automated stations using the M-Sorb-NK magnetic separation method;
• – a set of reagents for RT-PCR-RT analysis “CoV-2-FluA/B-RT-PCR”

Note: format 1 - lyophilized in flexible strips, for Rotor-Gene devices and plate cyclers or format 2 - lyophilized in PCR plates
Release form 2:

• - a set of reagents for RT-PCR-RT analysis “CoV-2-FluA/B-RT-PCR”
• Note: format 1 - lyophilized in flexible strips, for Rotor-Gene devices and plate cyclers or format 2 - lyophilized in PCR plates

5X Genta Taq-AB qPCR master mix is a ready-to–use mixture for qualitative or quantitative PCR with the ability to detect results in real time. 5X Genta Taq-AB qPCR Master Mix contains all the components necessary for PCR, including Genta Taq-AB polymerase, dNTP, Mg2+, SYBR Green I dye and reaction buffer. To set up the PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. Inactivation of Genta Taq-AB polymerase with antibodies allows preparation of mixtures at room temperature. The "hot" start provides an increase in the specificity of amplification. The dissociation of the complex and the release of Genta Taq-AB DNA polymerase is provided by heating above 70 °C.

ExtraGen®Black is a series of stable paramagnetic particles, ideal for the isolation of nucleic acids. ExtraGen® Black has a greater binding capacity compared to ExtraGen®, has a high binding efficiency with nucleic acids, is light and easy to use. ExtraGen® Black particles are stable under various conditions, which is especially important at the stages of sample preparation, in particular at the lysis stage. Characteristics and advantages: There is no need for a centrifuge and plastic columns High purity and speed of extraction Compatible with lysis/sequencing reagents and automated equipment

Genta RevM revertase (reverse transcriptase) is a genetically modified reverse transcriptase of the Moloney mouse leukemia virus (MMLV), designed for the synthesis of a complementary DNA chain (cDNA) on a single-stranded RNA matrix. Genta RevM was isolated from a strain of Escherichia coli expressing a modified MMLV reverse transcriptase gene. In comparison with the reverse transcriptase of the "wild type" Genta REV M, the revertase has increased thermal stability: the optimal temperature for the enzyme is 50 ° C, the enzyme remains active at temperatures up to 65 ° C. Reverse transcriptase Genta RevM revertase provides higher cDNA yield, allows longer fragments to be synthesized, has improved efficiency when using GC-rich RNA matrices, and also has higher productivity. The Genta RevM reverse transcriptase is suitable for RT-PCR in the "one-step" format.

Genta benzonuclease is a nuclease that cleaves all types of both DNA and RNA (single-stranded, double-stranded, linear and annular) to form oligodeoxynucleotides with terminal 5’-monophosphates 3-5 bases long. It is effective in a wide range of working conditions. It has no proteolytic activity. Isolated from a strain of Escherichia coli expressing the nuclease gene from the microorganism Serratia marcescens. Reaction conditions: 20 - 37 °C, optimal buffer: 50 mM Tris-HCl (pH 8.0 at 25 °C); 100 -150 mm NaCl; 1 mM MgCl2.

Genta TaqF DNA polymerase is a chemically modified recombinant analog of DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme has 5’-3’ polymerase activity and 5’-3’ exonuclease activity, but does not have 3’-5’ corrective exonuclease activity. The recombinant enzyme Genta TaqF DNA polymerase is suitable for use in routine PCR, including real-time PCR. Genta TaqF DNA polymerase is inactivated by chemical modification, which allows to prepare the reaction mixture at room temperature. The presence of a "hot start" increases the specificity and sensitivity of amplification. Polymerase activation occurs when it is heated to 95 ° C for 15 minutes.

5X Genta Single-tube RT-qPCR Master Mix is a ready-to–use mixture for conducting a cDNA synthesis reaction followed by quantitative and/or qualitative PCR with the possibility of detecting results in real time (RT-PCR-RV). 5X Genta Single-tube RT-qPCR Master Mix contains all the components necessary for RT-PCR, including Genta REV M reverse transcriptase, Genta TaqF DNA polymerase, dNTP, Mg2+, SYBR Green I dye and reaction buffer. To set up the RT-PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. The chemically inactivated DNA polymerase Genta TaqF allows to perform preparatory work at room temperature and provides highly specific amplification due to the possibility of a "hot" start. Inhibition of enzyme activity is removed when it is heated at 95 ° C for 15 minutes.

ExoGen is a ready-to-use mixture of Genta-ExoI exonuclease I enzymes and the thermolabile alkaline phosphatase Genta-ALP in a reaction buffer. ExoGen mixture is used for enzymatic hydrolysis of an excessive amount of primers and nucleotides in PCR products (amplicons). The PCR products purified in this way are suitable for further use in various biological applications, such as cloning and DNA sequencing.

The protocol is based on a modified method of alkaline lysis of a bacterial culture without preliminary sedimentation of bacteria, followed by selective binding of DNA from the clarified lysate on a siliconized column membrane. The technology makes it possible to speed up the isolation process due to rapid lysis directly from the biomass with treatment of the lysate with RNase A and further centrifugation.
Benefits of the set:
Yield up to 10 µg from 0.6 ml bacterial culture Indicators A260/280 = 1.80±0.05, A260/230 ≥ 2.1 It has been verified that plasmid DNA is suitable for restriction reactions, transformation, sequencing, PCR