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ExoGen is a ready-to-use mixture of Genta-ExoI exonuclease I enzymes and the thermolabile alkaline phosphatase Genta-ALP in a reaction buffer. ExoGen mixture is used for enzymatic hydrolysis of an excessive amount of primers and nucleotides in PCR products (amplicons). The PCR products purified in this way are suitable for further use in various biological applications, such as cloning and DNA sequencing.

Benefits of the set:

• DNA from any reaction mixtures, fragments from 70 to 20,000 bp in length.
• Indicators A260/280 = 1.80±0.05, A260/230 ≥ 2.0
• The isolated PCR product is suitable for ligation, restriction, transformation, sequencing, PCR.

Fluorescent probes are an oligonucleotide with a fluorescent label and a suppressor that can bind to the DNA chain during amplification. When the probe is in a free state, both dyes are located close to each other, therefore, fluorescence quenching occurs. During PCR, at the stage of chain elongation, the hybridized probe is cleaved by a polymerase that has 5’-exonuclease activity, the dyes are separated, and a fluorescent signal proportional to the amount of the amplified product is observed. The fluorescence intensity increases in each cycle in proportion to the probe splitting rate. The use of probes in PCR makes it possible to increase the accuracy and selectivity of the reaction. A wide range of fluorescent dyes: FAM, R6G, VIC, HEX, JOE, TAMRA, ROX, Cy 5 and Cy5.5 The possibility of multiplex analysis of multiple DNA targets in one test tube. High efficiency of PCR-RV. Probe quality control: HPLC purification and mass spectrometry verification

5x Genta TE is a buffer for the dilution of nucleic acids. Composition: 10 mM Tris*HCl, 1 mM EDTA, pH 8,0. Prepared with ultrapure water.

5X Genta qPCR Master Mix is a ready-to–use mixture for qualitative or quantitative PCR with real-time detection of results. The master mix ensures reliable PCR operation in a wide range of applications. The master mix allows PCR-RV to be carried out in a multiplex format. 5X Genta qPCR Master mix contains all the components necessary for PCR, including Genta TaqF polymerase, dNTP, Mg2+, SYBR Green I dye and reaction buffer. To set up the PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. The chemically inactivated DNA polymerase Genta TaqF allows to perform preparatory work at room temperature and provides highly specific amplification due to the possibility of a "hot" start. Inhibition of enzyme activity is removed when it is heated at 95 ° C for 15 minutes.

Genta TaqF DNA polymerase is a chemically modified recombinant analog of DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme has 5’-3’ polymerase activity and 5’-3’ exonuclease activity, but does not have 3’-5’ corrective exonuclease activity. The recombinant enzyme Genta TaqF DNA polymerase is suitable for use in routine PCR, including real-time PCR. Genta TaqF DNA polymerase is inactivated by chemical modification, which allows to prepare the reaction mixture at room temperature. The presence of a "hot start" increases the specificity and sensitivity of amplification. Polymerase activation occurs when it is heated to 95 ° C for 15 minutes.

5X Genta Taq-AB PCR Master mix is a ready-to–use mixture for qualitative or quantitative PCR with the ability to detect results in real time. 5X Genta Taq-AB PCR Master Mix contains all the components necessary for PCR, including Genta Taq-AB polymerase, dNTP, Mg2+ and reaction buffer. To set up the PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. Inactivation of Genta Taq-AB polymerase with antibodies allows preparation of mixtures at room temperature. The "hot" start provides an increase in the specificity of amplification. The dissociation of the complex and the release of Genta Taq-AB DNA polymerase is provided by heating above 70 °C.

The kit is designed for isolating plasmid DNA from bacteria.
The operating principle of the kit is based on alkaline lysis of RNase-treated biomaterial, followed by selective binding of DNA from the clarified lysate on a siliconized column membrane. The colored solutions in the kit allow you to control the correct order of adding components, the completeness of lysis and neutralization.
Benefits of the set:

• Yield up to 25 µg from 2 ml of bacterial culture and up to 60* µg from 6-10 ml of bacterial culture
• Indicators A260/280 = 1.80±0.05, A260/230 ≥ 2.1
• Plasmid DNA has been verified to be suitable for restriction reactions, transformation, sequencing, PCR, transfection.

*product yield depends on the copy number of the plasmid, culture volume and cultivation conditions

5X Genta Single-tube RT-PCR Master Mix is a ready-to–use mixture for conducting a cDNA synthesis reaction followed by quantitative and/or qualitative PCR with the possibility of detecting results in real time (RT-PCR-RV). 5X Genta Single-tube RT-PCR Master Mix contains all the components necessary for RT-PCR, including Genta REV M reverse transcriptase, Genta TaqF DNA polymerase, dNTP, Mg2+ and reaction buffer. To set up the RT-PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. The chemically inactivated DNA polymerase Genta TaqF allows for preparatory work at room temperature and provides highly specific amplification due to the possibility of a "hot" start. Inhibition of enzyme activity is removed when it is heated at 95 ° C for 15 minutes.

Release form 1:

• – a set of reagents for manual RNA isolation and at automated stations using the M-Sorb-NK magnetic separation method;
• – a set of reagents for RT-PCR-RT analysis “CoV-2-FluA/B-RT-PCR”

Note: format 1 - lyophilized in flexible strips, for Rotor-Gene devices and plate cyclers or format 2 - lyophilized in PCR plates
Release form 2:

• - a set of reagents for RT-PCR-RT analysis “CoV-2-FluA/B-RT-PCR”
• Note: format 1 - lyophilized in flexible strips, for Rotor-Gene devices and plate cyclers or format 2 - lyophilized in PCR plates

Genta Taq-AB DNA polymerase is a recombinant analog of DNA polymerase from thermophilic bacterium Thermus aquaticus inactivated by specific monoclonal antibodies. The enzyme has 5’-3’ polymerase activity and 5’-3’ exonuclease activity, but does not have 3’-5’ corrective exonuclease activity. The recombinant enzyme Genta Taq-AB DNA polymerase is suitable for use in routine PCR, including real-time PCR. Inactivation with monoclonal antibodies allows to prepare the reaction mixture at room temperature. The presence of a "hot start" increases the specificity and sensitivity of amplification. Dissociation of the complex with antibodies and activation of Genta Taq-AB DNA polymerase occurs when it is heated above 70 °C.

Genta benzonuclease is a nuclease that cleaves all types of both DNA and RNA (single-stranded, double-stranded, linear and annular) to form oligodeoxynucleotides with terminal 5’-monophosphates 3-5 bases long. It is effective in a wide range of working conditions. It has no proteolytic activity. Isolated from a strain of Escherichia coli expressing the nuclease gene from the microorganism Serratia marcescens. Reaction conditions: 20 - 37 °C, optimal buffer: 50 mM Tris-HCl (pH 8.0 at 25 °C); 100 -150 mm NaCl; 1 mM MgCl2.