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Release form 1:

• – a set of reagents for manual RNA isolation and at automated stations using the M-Sorb-NK magnetic separation method;
• – a set of reagents for RT-PCR-RT analysis “CoV-2-FluA/B-RT-PCR”

Note: format 1 - lyophilized in flexible strips, for Rotor-Gene devices and plate cyclers or format 2 - lyophilized in PCR plates
Release form 2:

• - a set of reagents for RT-PCR-RT analysis “CoV-2-FluA/B-RT-PCR”
• Note: format 1 - lyophilized in flexible strips, for Rotor-Gene devices and plate cyclers or format 2 - lyophilized in PCR plates

The kit “System for quantitative assessment of E. coli host DNA impurities by RT-PCR” is convenient and easy to use; it allows you to isolate residual DNA from a sample and amplify it using real-time PCR in the presence of the fluorescent dye SYBR Green I. The kit includes reagents for isolating DNA from nucleoprotein complexes and removing PCR inhibitors, a 2× reaction mixture BioMaster HS-qPCR SYBR Blue (2×), a standard DNA solution and a proven set of specific primers for the conserved region of E. coli 16S rRNA.

The kit is intended for the extraction and purification of DNA from the following samples: 1. Leaves, needles, stamens, green parts of plants 2. Roots, stems, bark 3. Fruits, berries, seeds 4. Mosses, lichens 5. Unicellular algae

Oligonucleotides are short fragments of nucleic acids that are widely used in various fields of DNA diagnostics (medical diagnostics, forensic analysis, veterinary medicine, food quality control, etc.). Recently, oligonucleotides have been widely used as medicines against various genetic and other diseases. GenTerra produces DNA and RNA oligonucleotides on a scale of several milligrams to gram quantities, including modified oligonuleotides, fluorescently labeled oligonucleotides and real-time PCR probes.

Fusion DNA polymerase is a recombinant polypeptide consisting of a fusion of the thermostable DNA polymerase of Pyrococcus furiosus (Pfu) and the DNA binding protein of the thermophilic archaea species Sulfolobus solfataricus (Sso7d). The Sso7d protein binds to the minor groove of double-stranded DNA and further stabilizes the polymerase-template complex. Due to this, Fusion DNA polymerase has increased processivity, synthesis accuracy, fragment amplification rate, and increased resistance to PCR inhibitors compared to native Pfu DNA polymerase [1]. Fusion DNA polymerase has 5'→3' polymerase activity, 3'→5' exonuclease activity and synthesizes blunt-ended products.
Fusion DNA polymerase is a good choice for routine cloning and can be used to generate long or complex amplicons by PCR.
Examples of using Fusion DNA polymerase are presented at the end of the description.
Fusion DNA polymerase was isolated from an E. coli strain containing a plasmid with a cloned DNA fragment consisting of the fusion genes of the thermostable DNA polymerase of Pyrococcus furiosus (Pfu) and the DNA-binding protein of Sulfolobus solfataricus (Sso7d).
One unit of activity corresponds to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble DNA fraction in 30 min at 74°C.