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Benefits of the set:

• DNA from any reaction mixtures, fragments from 70 to 20,000 bp in length.
• Indicators A260/280 = 1.80±0.05, A260/230 ≥ 2.0
• The isolated PCR product is suitable for ligation, restriction, transformation, sequencing, PCR.

The kit is designed for isolating plasmid DNA from bacteria.
The operating principle of the kit is based on alkaline lysis of RNase-treated biomaterial, followed by selective binding of DNA from the clarified lysate on a siliconized column membrane. The colored solutions in the kit allow you to control the correct order of adding components, the completeness of lysis and neutralization.
Benefits of the set:

• Yield up to 25 µg from 2 ml of bacterial culture and up to 60* µg from 6-10 ml of bacterial culture
• Indicators A260/280 = 1.80±0.05, A260/230 ≥ 2.1
• Plasmid DNA has been verified to be suitable for restriction reactions, transformation, sequencing, PCR, transfection.

*product yield depends on the copy number of the plasmid, culture volume and cultivation conditions

10-fold buffer for the ligation reaction, compatible with T4 DNA ligase.

Genta-ALP alkaline phosphatase thermolabile catalyzes the cleavage of 5’- and 3’-phosphate groups from DNA, RNA, oligonucleotides, nucleoside triphosphates, as well as dephosphorylation of proteins. Active in the PCR buffer. Isolated from a strain of Escherichia coli expressing the alkaline phosphatase gene from Gadus morua.

The specially formulated binding solution (GE) ensures effective cleaning with high yield. The kit is intended for scientific research only.
Benefits of the set:

• Yield 60-90%
• Indicators A260/280 = 1.80±0.05
• Purified DNA is suitable for ligation, restriction, transformation, sequencing, and PCR reactions.

The magnetic sorbent ExtraGen® is an aqueous suspension of solid-phase magnetic particles based on iron oxide and silicon oxide. It is designed to isolate nucleic acids from clinical and biological samples. The suspension of the magnetic sorbent has an "intermediate" sedimentation stability, which is especially important when using the material in automatic DNA isolation systems. ExtraGen® particles are resistant to heat, strong oxidants and intense mechanical influences (centrifugation, dispersion, etc.). The use of proprietary buffer solution allows the particles to be stored at room temperature for a year without deterioration of their sorption properties. As tests have shown, the ExtraGen® sorbent shows high efficiency in the isolation of nucleic acids both in manual mode and with the use of automatic stations.

The kit uses the technology of DNA sorption on magnetic particles. The entire DNA extraction process combines the steps of sample lysis, subsequent selective binding of the released DNA to magnetic beads, and then, after a series of washes, elution with the option of concentrating the DNA in a low-salt buffer.
Benefits of the set:

• High yield of genomic DNA from E.coli and eukaryotic cells: 4-5 µg DNA from 0.5-1*109 bacterial cells and 0.25-1*106 eukaryotic cells.
• High yield of genomic DNA when isolated from various mouse tissues and organs.
• Indicators A260/280 = 1.80±0.05, A260/230 ≥ 2.1
• Purified DNA is verified to be free of proteins, nucleases or other contaminants and can be used in a wide range of molecular biology techniques such as PCR, RT-PCR or other enzyme reactions.
• High throughput for DNA extraction from large numbers of samples.

A 5-fold reaction buffer that does not contain magnesium sulfate.

The dNTP mixture is an aqueous solution of four highly purified 2’-deoxynucleoside-5’-triphosphates (dATP, dTTP, dHTF, dCTF) in equimolar concentration

5x Genta TE is a buffer for the dilution of nucleic acids. Composition: 10 mM Tris*HCl, 1 mM EDTA, pH 8,0. Prepared with ultrapure water.

5X Genta qPCR Master Mix is a ready-to–use mixture for qualitative or quantitative PCR with real-time detection of results. The master mix ensures reliable PCR operation in a wide range of applications. The master mix allows PCR-RV to be carried out in a multiplex format. 5X Genta qPCR Master mix contains all the components necessary for PCR, including Genta TaqF polymerase, dNTP, Mg2+, SYBR Green I dye and reaction buffer. To set up the PCR reaction, only oligonucleotides, matrix and water must be added to the reaction mixture. The chemically inactivated DNA polymerase Genta TaqF allows to perform preparatory work at room temperature and provides highly specific amplification due to the possibility of a "hot" start. Inhibition of enzyme activity is removed when it is heated at 95 ° C for 15 minutes.

Genta TaqF DNA polymerase is a chemically modified recombinant analog of DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme has 5’-3’ polymerase activity and 5’-3’ exonuclease activity, but does not have 3’-5’ corrective exonuclease activity. The recombinant enzyme Genta TaqF DNA polymerase is suitable for use in routine PCR, including real-time PCR. Genta TaqF DNA polymerase is inactivated by chemical modification, which allows to prepare the reaction mixture at room temperature. The presence of a "hot start" increases the specificity and sensitivity of amplification. Polymerase activation occurs when it is heated to 95 ° C for 15 minutes.