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Genta Bst DNA polymerase is a large fragment of Bacillus stearothermophilus DNA polymerase (a 67 kDa polypeptide). The enzyme has 5’-3’ polymerase activity, but does not have 5’-3’ and 3’-5’ exonuclease activity. Genta Bst DNA polymerase has a displacing activity and can be used for isothermal amplification of DNA in the LAMP/RT-LAMP format. The optimal temperature of enzyme activity is 60-65 °C.

A 5-fold reaction buffer that does not contain magnesium sulfate.

A 5-fold reaction buffer suitable for use in the reaction of isothermal amplification of nucleic acids in LAMP and OT-LAMP formats. The standard 5x Genta LAMP buffer contains 25 mm MgSO4, which corresponds to 5 mM MgSO4 in the reaction mixture.

Genta UDG thermolabile glycosylase is a thermosensitive enzyme that catalyzes the release of uracil from uracil-containing single- or double-stranded DNA. It is used to prevent the appearance of false positive results caused by contamination with amplicons. The enzyme does not show activity against RNA and oligomers and is fully incubated during the first PCR cycle when heated above 70 ° C, without interfering with the amplification of PCR products.

Buffer for storage and dilution of DNA polymerase.

Genta-T4 DNA ligase is a recombinant enzyme that catalyzes the formation of a phosphodiester bond between the 5`-phosphate and 3`-hydroxyl end groups of double-stranded DNA. Isolated from a strain of Escherichia coli expressing the bacteriophage T4 DNA ligase gene. DNA ligase stitches both sticky and blunt ends, can repair single-strand breaks in double strands of DNA, RNA or DNA/RNA hybrids.

Buffer for storage and dilution of reverse transcriptase.

Genta Taq-AB DNA polymerase is a recombinant analog of DNA polymerase from thermophilic bacterium Thermus aquaticus inactivated by specific monoclonal antibodies. The enzyme has 5’-3’ polymerase activity and 5’-3’ exonuclease activity, but does not have 3’-5’ corrective exonuclease activity. The recombinant enzyme Genta Taq-AB DNA polymerase is suitable for use in routine PCR, including real-time PCR. Inactivation with monoclonal antibodies allows to prepare the reaction mixture at room temperature. The presence of a "hot start" increases the specificity and sensitivity of amplification. Dissociation of the complex with antibodies and activation of Genta Taq-AB DNA polymerase occurs when it is heated above 70 °C.

5x Genta TE is a buffer for the dilution of nucleic acids. Composition: 10 mM Tris*HCl, 1 mM EDTA, pH 8,0. Prepared with ultrapure water.

ExtraGen®Black is a series of stable paramagnetic particles, ideal for the isolation of nucleic acids. ExtraGen® Black has a greater binding capacity compared to ExtraGen®, has a high binding efficiency with nucleic acids, is light and easy to use. ExtraGen® Black particles are stable under various conditions, which is especially important at the stages of sample preparation, in particular at the lysis stage. Characteristics and advantages: There is no need for a centrifuge and plastic columns High purity and speed of extraction Compatible with lysis/sequencing reagents and automated equipment

Genta RevM revertase (reverse transcriptase) is a genetically modified reverse transcriptase of the Moloney mouse leukemia virus (MMLV), designed for the synthesis of a complementary DNA chain (cDNA) on a single-stranded RNA matrix. Genta RevM was isolated from a strain of Escherichia coli expressing a modified MMLV reverse transcriptase gene. In comparison with the reverse transcriptase of the "wild type" Genta REV M, the revertase has increased thermal stability: the optimal temperature for the enzyme is 50 ° C, the enzyme remains active at temperatures up to 65 ° C. Reverse transcriptase Genta RevM revertase provides higher cDNA yield, allows longer fragments to be synthesized, has improved efficiency when using GC-rich RNA matrices, and also has higher productivity. The Genta RevM reverse transcriptase is suitable for RT-PCR in the "one-step" format.

Genta benzonuclease is a nuclease that cleaves all types of both DNA and RNA (single-stranded, double-stranded, linear and annular) to form oligodeoxynucleotides with terminal 5’-monophosphates 3-5 bases long. It is effective in a wide range of working conditions. It has no proteolytic activity. Isolated from a strain of Escherichia coli expressing the nuclease gene from the microorganism Serratia marcescens. Reaction conditions: 20 - 37 °C, optimal buffer: 50 mM Tris-HCl (pH 8.0 at 25 °C); 100 -150 mm NaCl; 1 mM MgCl2.